Overview
| 英文名称 | Mouse EG-VEGF(Endocrine Gland Derived Vascular Endothelial Growth Factor) ELISA Kit |
| 别名 | PK1, EGVEGF, PRK1, PROK1, Prokineticin 1, Black Mamba Toxin-Related Protein, Mambakine |
| UniProt ID | Q14A28 |
| 种属 | Mouse |
| 研究领域 | Cytokine;Tumor immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 5.8 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EG-VEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EG-VEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EG-VEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EG-VEGF in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
