Overview
| 英文名称 | Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit |
| 别名 | ENPP7, E-NPP 7, NPP-7, Ectonucleotide Pyrophosphatase/Phosphodiesterase 7, Sphingomyelin Phosphodiesterase, Intestinal alkaline sphingomyelinase |
| UniProt ID | Q6UWV6 |
| 种属 | Human |
| 研究领域 | Enzyme & Kinase;Neuro science; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.109 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
