Overview
| 英文名称 | Chicken IL-12p40(Interleukin 12 p40) ELISA Kit |
| 别名 | IL-12B, p40, IL12B, CLMF2, NKSF2, Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, Interleukin 23, P40 Subunit |
| 种属 | Chicken |
| 研究领域 | Cytokine;Infection immunity;Immune molecule; |
| 检测类型 | Sandwich |
| 灵敏度 | 5.9 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma and other biological fluids. |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
