Overview
| 英文名称 | Human PK2(Prokineticin 2) ELISA Kit |
| 别名 | PK-2, Bv8, MIT1, KAL4, Protein Bv8 Homolog |
| UniProt ID | Q9HC23 |
| 种属 | Human |
| 研究领域 | Neuro science; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 95.3 pg/mL |
| 标准品 | 20000 pg/mL |
| 检测范围 | 312.5-20000 pg/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PK2 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
