Overview
| 英文名称 | Human GRP78(Glucose Regulated Protein 78) ELISA Kit |
| 别名 | MIF2, BIP, GRP78, Immunoglobulin heavy chain-binding protein, Glucose Regulated Protein 78, Binding Immunoglobulin Protein, Endoplasmic reticulum lumenal Ca binding grp78, Heat Shock 70kDa Protein 5, HSPA5 |
| UniProt ID | P11021 |
| 种属 | Human |
| 研究领域 | Signal transduction;Tumor immunity;Infection immunity; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.133 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GRP78. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GRP78. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GRP78, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GRP78 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
