Overview
| 英文名称 | Human GIF(Gastric Intrinsic Factor) ELISA Kit |
| 别名 | TCN3, IF, IFMH, INF, Transcobalamin III, Vitamin B Synthesis |
| UniProt ID | P27352 |
| 种属 | Human |
| 研究领域 | Endocrinology; |
| 检测类型 | Competitive Inhibition |
| 灵敏度 | 0.91 ng/mL |
| 标准品 | 200 ng/mL |
| 检测范围 | 3.13-200 ng/mL |
| 样本类型 | Serum, plasma and other biological fluids |
| 检测时间 | 2h |
检测原理
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
