Overview
| 英文名称 | Rat NPHN(Nephrin) ELISA Kit |
| 别名 | NPHS1, CNF, Nephrosis 1, Congenital Finnish Type, Renal glomerulus-specific cell adhesion receptor |
| UniProt ID | Q9R044 |
| 种属 | Rat |
| 研究领域 | Tumor immunity;Kidney biomarker;Urology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.127 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NPHN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NPHN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NPHN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NPHN in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
