Overview
| 英文名称 | Human NAP(Neutrophil Alkaline Phosphatase) Microsample ELISA Kit |
| 别名 | LAP, Leukocyte Alkaline Phosphatase |
| 种属 | Human |
| 研究领域 | Enzyme & Kinase;Tumor immunity;Infection immunity;Hematology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.52 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NAP in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
