Overview
| 英文名称 | EasyStep Human IL6(Interleukin 6) ELISA Kit |
| 别名 | IL-6,MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor |
| UniProt ID | P05231 |
| 种属 | Human |
| 研究领域 | Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.1 pg/mL |
| 标准品 | 100 pg/mL |
| 检测范围 | 1.56-100 pg/mL |
| 样本类型 | serum, plasma |
| 检测时间 | 1.5 h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6, and the Human IL6 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL6. After TMB substrate solution is added, only those wells that contain Human IL6 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
