Overview
| 英文名称 | EasyStep Human OC(Osteocalcin) ELISA Kit |
| 别名 | BGLAP, OT, BGP, Bone Gla Protein, Bone Gamma-Carboxyglutamate Protein, Gamma-carboxyglutamic acid-containing protein |
| UniProt ID | P02818 |
| 种属 | Human |
| 研究领域 | Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.14 ng/mL |
| 标准品 | 15 ng/mL |
| 检测范围 | 0.23-15 ng/mL |
| 样本类型 | Serum |
| 检测时间 | 1.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OC. After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
