Overview
| 英文名称 | Cattle cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit |
| 别名 | CMH2, cTnT, TnTc, Cardiomyopathy,Hypertrophic 2, Cardiac muscle troponin T, TNNT2, Troponin T Type 2, Cardiac |
| UniProt ID | P13789 |
| 种属 | Cattle |
| 研究领域 | Cardiovascular biology; |
| 检测类型 | Sandwich |
| 灵敏度 | 26.1 pg/mL |
| 标准品 | 5000 pg/mL |
| 检测范围 | 78.13-5000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
