Overview
| 英文名称 | Rat HbA1c(Glycated Hemoglobin A1c) ELISA Kit |
| 别名 | Glycosylated Hemoglobin, Hemoglobin A1c, Hb1c, HbAIc, HbAIc |
| 种属 | Rat |
| 研究领域 | Diagnosis of diabetes mellitus |
| 检测类型 | Sandwich |
| 灵敏度 | 4.25 µg/mL |
| 标准品 | 1000 µg/mL |
| 检测范围 | 15.63-1000 µg/mL |
| 样本类型 | plasma and erythrocyte lysates |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HbA1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
