Overview
| 英文名称 | Dog CA125(Carbohydrate Antigen 125) ELISA Kit |
| 别名 | MUC16, Cancer Antigen 125, Mucin 16,Cell Surface Associated, Ovarian Cancer-Related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125 |
| 种属 | Dog |
| 研究领域 | Tumor immunity;Reproductive science; |
| 检测类型 | Sandwich |
| 灵敏度 | 0.61 U/mL |
| 标准品 | 100 U/mL |
| 检测范围 | 1.57-100 U/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CA125. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CA125. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CA125, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CA125 in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
