Overview
| 英文名称 | Cattle NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit |
| 别名 | NT-Pro-BNP, N-BNP, ANFB_HUMAN, BNP(1-32), BNP(5-29), BNP-32, Gamma-brain natriuretic peptide, nppb |
| 种属 | Cattle |
| 研究领域 | Endocrinology;Cardiovascular biology; |
| 检测类型 | Sandwich |
| 灵敏度 | 12 pg/mL |
| 标准品 | 2500 pg/mL |
| 检测范围 | 39.07-2500 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 检测时间 | 3.5h |
检测原理
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.
标准曲线
