Overview
| 别名 | FIB; FLRN; Nop1; RNU3IP1; Fibrillarin/U3 RNP; 34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; Fbl; |
| 基因名 | FBL |
| UniProt ID | P22087 |
| 反应种属 | Human,Mouse,Rat |
| 应用 | WB,IHC-P,ICC/IF,FC |
| 宿主 | Rabbit |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG |
| 克隆号 | R05-6L-7 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 34 kDa; Observed MW: 34 kDa |
| 纯化方式 | Affinity Chromatography |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000-1:5000; ICC/IF-1:500-1:5000; FC-1:10-1:100; IHC-1:50-1:100 |
| 存储缓冲液 | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place. Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence. The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development. |
检测原理