Overview
| 别名 | TK1; Thymidine kinase 1; EC 2.7.1.21; KITH; TK-1; cytosolic |
| 基因名 | TK1 |
| UniProt ID | P04183 |
| 反应种属 | Human |
| 应用 | WB,IHC-P,FC,IP |
| 宿主 | Rabbit |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG |
| 克隆号 | R03-4X-4 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| 纯化方式 | Affinity Chromatography |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:500-1:1000; IHC-P-1:100-1:200; FC-1:100-1:200; IP-1:20 |
| 存储缓冲液 | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. |
检测原理