![[KD]Cortactin Rabbit mAb-抗体-威格顿生物](/uploads/20260414/ad4291519985458ac81d7dea66c4005d.png)
![[KD]Cortactin Rabbit mAb](/assets/addons/ldcms/ldcms2025/images/product-default-flat.png)
Overview
| 别名 | CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1 |
| 基因名 | CTTN |
| UniProt ID | Q14247 |
| 反应种属 | Human,Mouse,Rat |
| 应用 | WB,IHC-P,ICC/IF,FC,IP |
| 宿主 | Rabbit |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG |
| 克隆号 | R01-9T-5 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 62 kDa; Observed MW: 62-80 kDa |
| 纯化方式 | Affinity Chromatography |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000-1:5000; IHC-P-1:100-1:200; ICC/IF-1:500-1:1000; FC-1:20; IP-1:20-1:50 |
| 存储缓冲液 | Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks. |
检测原理