Overview
| 别名 | NAD kinase; Poly(P)/ATP NAD kinase |
| 基因名 | NADK |
| UniProt ID | O95544 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 9K5-U6-U4 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 49 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:500 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | NAD kinase (NADK) is a critical enzyme that phosphorylates nicotinamide adenine dinucleotide (NAD⁺) to produce NADP⁺, which is subsequently reduced to NADPH, a vital cofactor in anabolic reactions and redox homeostasis. Structurally, NADK contains conserved domains including the GGDG motif, NE/D short motif, and a conserved region II, which are essential for NAD⁺ binding and catalytic activity. Key residues such as D185, N280, E281, T321, and Y323 play crucial roles in substrate recognition and phosphorylation. Mammalian NADK typically forms a homotetramer, with phosphorylation sites regulated by kinases like Akt and PKC, enabling dynamic control of its activity. NADK's function is evolutionarily conserved across species, underscoring its fundamental role in cellular metabolism. Clinically, mutations in NADK or its mitochondrial isoform NADK2 cause severe metabolic and developmental disorders, including mitochondrial dysfunction, hyperlysinemia, and neurological defects. Additionally, NADK dysregulation is linked to cancer progression due to enhanced NADP(H)-dependent antioxidant defenses. |
检测原理