Overview
| 别名 | Purine nucleoside phosphorylase; PNP; Inosine phosphorylase; Inosine-guanosine phosphorylase |
| 基因名 | PNP |
| UniProt ID | P00491 |
| 反应种属 | Human |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 4B1-G6-H6 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 32 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Purine nucleoside phosphorylase (PNP) is a homotrimeric enzyme of the NP-I family that catalyzes the reversible phosphorolysis of purine and 2′-deoxypurine nucleosides, generating free purine bases and alpha-D-(deoxy)ribose-1-phosphate, thereby supporting the purine salvage and degradation pathways in many organisms. In humans, PNP shows preference for 6‑oxopurine ribonucleosides like inosine and guanosine, and its active site employs key residues to bind phosphate and stabilize the glycosidic bond–cleavage transition state. Structural and mutagenesis studies demonstrate that substitutions at these catalytic residues can drastically alter substrate specificity and catalytic efficiency, and engineered variants are being explored for biotechnological uses such as lowering purine content in foods. Inherited PNP deficiency, caused by biallelic pathogenic variants in the PNP gene (14q11.2), leads to toxic accumulation of purine metabolites, thymic apoptosis, and profound T‑cell lymphopenia, producing a rare autosomal recessive primary immunodeficiency that ranges from severe combined immunodeficiency to combined immunodeficiency with recurrent infections, autoimmunity, and neurologic impairment. |
检测原理