Overview
| 别名 | Bisphosphoglycerate mutase; BPGM; 2; 3-bisphosphoglycerate mutase; erythrocyte; 2; 3-bisphosphoglycerate synthase; 2; 3-diphosphoglycerate mutase; DPGM; BPG-dependent PGAM |
| 基因名 | BPGM |
| UniProt ID | P07738 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 4Y1-H3-O5 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 30 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Bisphosphoglycerate mutase (BPGM) is a multifunctional enzyme primarily expressed in erythrocytes and placental cells. It plays a central role in regulating hemoglobin oxygen affinity by catalyzing the synthesis of 2,3-bisphosphoglycerate (2,3-BPG) from 1,3-bisphosphoglycerate. As an allosteric effector, 2,3-BPG binds to hemoglobin, facilitating the release of oxygen to tissues and supporting fetal-maternal oxygen transfer. BPGM also possesses weaker mutase and phosphatase activities that regulate the degradation of 2,3-BPG. The enzyme functions as a homodimer, utilizing key active-site residues like His11 to facilitate phosphotransfer. Mutations in the BPGM gene are clinically linked to hemolytic anemia and familial erythrocytosis 8, conditions resulting from abnormally high oxygen affinity and subsequent compensatory erythropoiesis. |
检测原理