Overview
| 别名 | Myosin light chain 3; Cardiac myosin light chain 1; CMLC1; Myosin light chain 1; slow-twitch muscle B/ventricular isoform; MLC1SB; Ventricular myosin alkali light chain; Ventricular myosin light chain 1; VLCl; Ventricular/slow twitch myosin alkali light chain; MLC-lV/sb |
| 基因名 | MYL3 |
| UniProt ID | P08590 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 8I4-R3-S9 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 21 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Myosin light chain 3 (MYL3) is a regulatory light chain of myosin primarily expressed in cardiac ventricular and slow-twitch skeletal muscle. It is a fundamental component of the sarcomere, where it binds to the neck region of the myosin heavy chain to stabilize the long alpha-helical lever arm. This stabilization is crucial for modulating myosin crossbridge kinetics and the affinity of myosin for actin, thereby regulating muscle contraction. Although MYL3 belongs to the EF-hand superfamily, it does not bind calcium under physiological conditions. Mutations in the MYL3 gene are well-established causes of familial hypertrophic cardiomyopathy, particularly the mid-left ventricular chamber type. These pathogenic variants disrupt the structural integrity of the myosin head, leading to impaired cardiac function and compensatory hypertrophy, making MYL3 a significant marker in clinical genetic screening for inherited heart disease. |
检测原理