Overview
| 别名 | cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1 |
| 基因名 | PKA RI alpha |
| UniProt ID | P10644 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 5T1-R9-B3 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 42 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:200-1:250 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Protein kinase cAMP-dependent type I regulatory subunit alpha (PKA RI alpha) is a critical component of the protein kinase A (PKA) holoenzyme, encoded by the PRKAR1A gene. It functions as a regulatory subunit that binds and inhibits PKA catalytic subunits in the absence of cAMP. Upon cAMP binding, the tetrameric complex dissociates, releasing active catalytic subunits to phosphorylate targets involved in lipid and glucose metabolism, cell growth, and division. Beyond its role in cAMP-mediated signaling, it acts as a tissue-specific extinguisher and utilizes a nonconventional nuclear localization sequence to assist in DNA replication via RFC40 transport. Mutations in PRKAR1A are well-established causes of Carney complex type 1 (CNC1), an autosomal dominant syndrome characterized by cardiac myxomas, endocrine tumors, and primary pigmented nodular adrenocortical disease. It is also linked to acrodysostosis 1 and certain thyroid tumors. |
检测原理