Overview
| 别名 | DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme |
| 基因名 | Topoisomerase II alpha |
| UniProt ID | P11388 |
| 反应种属 | Human |
| 应用 | WB,IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 1P3-A1-A4 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 174 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000; IHC-1:250-1:500 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | DNA topoisomerase II alpha (Topoisomerase II alpha) is an essential nuclear enzyme that modulates DNA topology during critical cellular processes, including transcription, replication, and chromosome segregation. Functioning as a homodimer, the enzyme utilizes ATP hydrolysis to generate transient double-strand DNA breaks via a phosphotyrosine linkage at Tyr782. This mechanism allows for the passage of a second DNA duplex through the break to relieve torsional stress and enable decatenation before religation. The protein contains an N-terminal ATPase domain, a central TOPRIM domain for magnesium binding, and a C-terminal region for nuclear localization. Topoisomerase II alpha is indispensable for cell proliferation and is a primary target for anticancer agents such as etoposide. Clinically, its overexpression is associated with gliomas, while mutations or reduced activity are linked to drug resistance and ataxia-telangiectasia. |
检测原理