Overview
| 别名 | Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox |
| 基因名 | NCF1 |
| UniProt ID | P14598 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2a |
| 克隆号 | 2M5-B9-L7 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 44 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Neutrophil cytosolic factor 1 (NCF1), also known as p47phox, is a vital cytosolic subunit of the NADPH oxidase complex in phagocytes. Upon activation, NCF1 is phosphorylated and translocates to the plasma membrane, where it binds the cytochrome b558 complex to assemble the functional enzyme. This complex mediates the transfer of electrons to molecular oxygen, generating the superoxide anions required for the respiratory burst and pathogen clearance. Clinically, mutations in the NCF1 gene are a primary cause of chronic granulomatous disease (CGD), an immunodeficiency characterized by the inability to produce reactive oxygen species. Furthermore, genetic variability in NCF1 is well-established as a susceptibility factor for various autoimmune conditions, including rheumatoid arthritis, systemic lupus erythematosus, and Sjögren's syndrome, while reduced levels are linked to altered vascular function and hypertension. |
检测原理