Overview
| 别名 | Wilms tumor protein; WT33 |
| 基因名 | Wilms Tumor Protein |
| UniProt ID | P19544 |
| 反应种属 | Human |
| 应用 | WB,IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2a |
| 克隆号 | 3C8-C6-G5 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 49 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000; IHC-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | WT1 transcription factor (Wilms Tumor Protein) is a versatile zinc finger protein that acts as a master regulator of urogenital development, particularly in kidney mesenchyme differentiation. It contains four C-terminal C2H2-type zinc fingers for DNA and RNA binding and an N-terminal domain that mediates both transcriptional activation and repression. WT1 modulates the expression of genes involved in the mesenchymal-to-epithelial transition (MET), apoptosis, and angiogenesis, while also stabilizing specific mRNAs. It exists in multiple isoforms that vary in their binding affinity and post-transcriptional roles. Clinically, WT1 is famously associated with Wilms tumor, a pediatric kidney malignancy. Paradoxically, the protein can function as either a tumor suppressor or an oncogene depending on the cellular context. Mutations or dysregulation of WT1 disrupt normal nephrogenesis, leading to the rapid proliferation of embryonic rests and tumorigenesis. |
检测原理