Overview
| 别名 | Carbonic anhydrase 5A; mitochondrial; Carbonate dehydratase VA; Carbonic anhydrase VA; CA-VA |
| 基因名 | CA5A |
| UniProt ID | P35218 |
| 反应种属 | Human,Rat |
| 应用 | WB,ELISA |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 6T4-U8-R3 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 34 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000; ELISA-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Carbonic anhydrase 5A (CA5A) is a mitochondrial enzyme that plays a crucial role in maintaining acid-base balance by catalyzing the reversible hydration of carbon dioxide to bicarbonate. This reaction is vital for various metabolic processes, particularly in the liver, where CA5A supports other enzymes involved in energy production and the urea cycle, which detoxifies ammonia. The enzyme's properties include a high catalytic efficiency and a unique active site structure compared to other carbonic anhydrases, involving cysteine residues instead of histidine. Deficiencies in CA5A due to mutations in the CA5A gene can lead to carbonic anhydrase VA deficiency, a rare inherited disorder characterized by severe metabolic crises, including poor feeding, rapid breathing, and seizures, particularly in infancy. |
检测原理