Overview
| 别名 | Transaldolase |
| 基因名 | TALDO1 |
| UniProt ID | P37837 |
| 反应种属 | Human |
| 应用 | WB,IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2a |
| 克隆号 | 7C1-D7-W8 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 37 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000; IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Transaldolase 1 (TALDO1) is a cytosolic enzyme crucial for the non-oxidative phase of the pentose phosphate pathway, where it catalyzes the reversible transfer of a three-carbon dihydroxyacetone unit from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing erythrose-4-phosphate and fructose-6-phosphate. Structurally, human transaldolase consists of 337 amino acids forming a single α/β barrel domain, with the active site containing lysine-142, glutamate-106, and aspartate-27, which are essential for its catalytic mechanism involving Schiff base formation and proton transfer. The enzyme functions as a dimer and is vital for generating ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. Deficiency or mutations in TALDO1 can disrupt the pentose phosphate pathway, leading to transaldolase deficiency, a rare metabolic disorder characterized by liver dysfunction, coagulopathy, and multi-organ involvement due to impaired redox balance and nucleotide synthesis. |
检测原理