Overview
| 别名 | Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF |
| 基因名 | NSF |
| UniProt ID | P46459 |
| 反应种属 | Human |
| 应用 | WB,IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 8B6-R2-H3 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 82 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000; IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | N-ethylmaleimide sensitive factor, vesicle fusing ATPase (NSF) is a ubiquitously expressed cytosolic AAA+ ATPase that functions as a homohexamer to drive intracellular membrane trafficking. NSF plays a fundamental role in the vesicle fusion cycle by binding to SNARE complexes via soluble NSF attachment proteins (SNAPs). Utilizing energy from ATP hydrolysis, it mechanically disassembles post-fusion cis-SNARE complexes, thereby recycling individual SNARE proteins for subsequent rounds of fusion. This chaperone-like activity is essential for nearly all trafficking events, including synaptic vesicle exocytosis and the transport of AMPA receptors. Consequently, NSF is a critical determinant of neurotransmitter release and synaptic plasticity. Clinically, dysfunction in NSF-mediated SNARE disassembly is implicated in various neurological disorders characterized by defects in synaptic transmission and cognitive function. |
检测原理