Overview
| 别名 | N-acylethanolamine-hydrolyzing acid amidase; Acid ceramidase-like protein; Acylsphingosine deacylase NAAA; N-acylsphingosine amidohydrolase-like; ASAH-like protein) [Cleaved into: N-acylethanolamine-hydrolyzing acid amidase subunit alpha; N-acylethanolamine-hydrolyzing acid amidase subunit beta] |
| 基因名 | NAAA |
| UniProt ID | Q02083 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 5E6-F6-I7 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 40 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:200-1:250 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | N-acylethanolamine acid amidase (NAAA) is an N-terminal cysteine hydrolase primarily localized within the lysosomes of immune cells, such as macrophages. It functions optimally at an acidic pH to catalyze the hydrolysis of bioactive fatty acid ethanolamides, with a high preference for N-palmitoylethanolamine (PEA). By degrading PEA, NAAA deactivates its anti-inflammatory and analgesic signaling, which is typically mediated through the peroxisome proliferator-activated receptor-alpha (PPAR-alpha). The catalytic mechanism of NAAA involves a nucleophilic cysteine residue and requires N-glycosylation for full enzymatic activity. Unlike other amidases, NAAA specifically targets short, unsaturated N-acylethanolamines. Clinically, NAAA is a significant therapeutic target for the treatment of inflammatory and neuropathic pain; its inhibition leads to elevated PEA levels, thereby enhancing endogenous antinociceptive and anti-inflammatory responses. |
检测原理