Overview
| 别名 | TP53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1 |
| 基因名 | 53BP1 |
| UniProt ID | Q12888 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 2R7-A9-N2 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 213 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Tumor protein p53 binding protein 1 (TP53BP1) is a crucial protein involved in DNA double-strand break repair, particularly in promoting non-homologous end joining (NHEJ) pathways. It interacts with damaged chromatin, recognizing specific epigenetic markers, and plays a role in telomere dynamics and class-switch recombination during antibody genesis. TP53BP1 forms a complex with RIF1, inhibiting DNA end resection and competing with BRCA1-CtIP to determine the repair pathway choice. The protein contains multiple functional domains and exists in three cellular pools: cytoplasmic, nuclear, and DNA repair foci. TP53BP1 also binds to the p53 protein, regulating cell cycle progression and proliferation. In terms of disease relevance, TP53BP1 is underexpressed in most cases of triple-negative breast cancer. Additionally, as individuals age, the recruitment of 53BP1 to double-strand breaks during the G1 phase becomes deficient, potentially contributing to the aging process through error-prone repair mechanisms. |
检测原理