Overview
| 别名 | Vacuolar protein sorting-associated protein 53 homolog |
| 基因名 | VPS53 |
| UniProt ID | Q5VIR6 |
| 反应种属 | Human,Mouse |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2a |
| 克隆号 | 9J5-S7-G2 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 94 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:2000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | VPS53 subunit of GARP complex (VPS53) is a core component of the Golgi-associated retrograde protein (GARP) complex, a multisubunit assembly that tethers vesicles for retrograde trafficking from endosomes to the trans-Golgi network (TGN). It interacts with small GTPases like Rab6 and SNARE proteins such as syntaxin 6 and 16 to facilitate membrane fusion and cargo delivery. Structurally, VPS53 utilizes C-terminal alpha-helical bundles for vesicle recognition. The depletion of VPS53 disrupts the localization and stability of Golgi enzymes, leading to significant defects in N- and O-glycosylation and glycoprotein processing. Functionally, VPS53 is essential for maintaining cellular lipid homeostasis and proper Golgi architecture. Its loss is associated with severe cellular phenotypes, including neuronal dendritic defects characterized by reduced branching and length, highlighting its importance in maintaining the structural integrity of the secretory pathway. |
检测原理