Overview
| 别名 | E3 ubiquitin-protein ligase RNF220; RING finger protein 220; RING-type E3 ubiquitin transferase RNF220 |
| 基因名 | RNF220 |
| UniProt ID | Q5VTB9 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2b |
| 克隆号 | 2B9-C3-T3 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 62 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Ring finger protein 220 (RNF220) is an evolutionarily conserved RING-type E3 ubiquitin-protein ligase that is highly expressed in the nervous system. It localizes primarily to the nuclear lamina and regulates signal transduction through both ubiquitin-dependent degradation and protein stabilization. RNF220 targets SIN3B for proteasomal degradation and, independently of its ligase activity, stabilizes β-catenin by recruiting the deubiquitinase USP7, thereby promoting canonical Wnt signaling. It also modulates the Sonic hedgehog (Shh)/Gli and interferon-STAT1 pathways, which are critical for neural patterning and motor neuron development. Clinically, pathogenic variants in RNF220 are associated with hypomyelinating leukodystrophy 23, a disorder characterized by ataxia, deafness, and dilated cardiomyopathy. Its broad influence on developmental signaling pathways also links RNF220 to tumorigenesis and immune dysregulation, reflecting its essential role in cellular homeostasis and neural integrity. |
检测原理