Overview
| 别名 | E3 ubiquitin-protein ligase TRIM65; Tripartite motif-containing protein 65 |
| 基因名 | TRIM65 |
| UniProt ID | Q6PJ69 |
| 反应种属 | Human |
| 应用 | WB,IP,CHIP |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2b |
| 克隆号 | 8R4-H5-J6 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 57 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000; IP-1:100; CHIP-1:100 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | TRIM65, a member of the tripartite motif (TRIM) protein family, functions primarily as an E3 ubiquitin ligase and is implicated in various diseases, particularly cancers and inflammatory conditions. Research indicates that TRIM65 promotes tumor progression by ubiquitinating key proteins involved in cell cycle regulation and apoptosis, such as Axin1 and p53, thereby facilitating the growth of cancers like renal cell carcinoma and colorectal cancer. Additionally, TRIM65 plays a significant role in innate immunity, particularly in the regulation of the NLRP3 inflammasome, where it negatively regulates its activation through ubiquitination, thus influencing inflammatory responses in diseases like atherosclerosis and gout. Despite its involvement in these critical biological processes, the full scope of TRIM65's functions and mechanisms remains underexplored, highlighting the need for further research to elucidate its potential as a therapeutic target in various diseases. |
检测原理