Overview
| 别名 | Putative uncharacterized protein FLJ43944 |
| 基因名 | YQ015 |
| UniProt ID | Q6ZRG5 |
| 反应种属 | Human,Mouse |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 6U8-H2-U4 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 24 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | YQ015 is a recombinant human protein expressed in E. coli or 293F systems, likely related to de novo designed self-assembling peptides like the Y15 variant, which forms amphiphilic β-sheet nanofibers through hydrophobic Tyr interactions, π–π stacking, electrostatic Glu-Lys bonds, and hydrogen bonding on the main chain . This peptide exhibits strong assembly propensity in aqueous solutions and cellular contexts, with critical aggregation concentrations around 50 nM for Y15-tagged superfolder green fluorescent protein (sfGFP), forming irreversible solid-like fibrous structures less sensitive to ionic strength up to 500 mM NaCl; its hydrophobic interfaces remain dry, distinguishing it from hydrophilic liquid-liquid phase separation motifs like FUS LC domains . These properties enable bottom-up synthesis of intracellular supramolecules and guide-tag systems for client protein enrichment. |
检测原理