Overview
| 别名 | Pyridoxine-5'-phosphate oxidase; Pyridoxamine-phosphate oxidase |
| 基因名 | PNPO |
| UniProt ID | Q9NVS9 |
| 反应种属 | Human |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 8K4-P8-Z7 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 29 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Pyridoxamine 5'-phosphate oxidase (PNPO) is a homodimeric enzyme essential for vitamin B6 metabolism, catalyzing the oxidation of pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP), the active form of vitamin B6. Structurally, each subunit features a split-barrel motif composed of both alpha-helices and beta-sheets, stabilized by disulfide bonds and salt-bridge interactions, and binds one PLP molecule tightly. The enzyme requires flavin mononucleotide (FMN) as a cofactor, which is held in a deep cleft between the subunits and is crucial for substrate binding and catalysis. The active site undergoes conformational changes upon substrate binding, shifting from an open to a partially closed state to facilitate PLP production. Mutations in the PNPO gene can lead to neonatal epileptic encephalopathy, a severe neurological disorder characterized by early-onset seizures due to impaired PLP synthesis. |
检测原理