Overview
| 别名 | E3 ubiquitin-protein ligase RNF216; RING finger protein 216; RING-type E3 ubiquitin transferase RNF216; Triad domain-containing protein 3; Ubiquitin-conjugating enzyme 7-interacting protein 1; Zinc finger protein inhibiting NF-kappa-B |
| 基因名 | RNF216 |
| UniProt ID | Q9NWF9 |
| 反应种属 | Human,Mouse |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2a |
| 克隆号 | 7K1-V5-X8 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 99 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:2000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Ring finger protein 216 (RNF216) is an E3 ubiquitin ligase that serves as a critical regulator of antiviral immunity, synaptic plasticity, and male fertility. It functions by transferring ubiquitin to target substrates for proteasomal degradation, thereby modulating various signaling pathways. In the context of innate immunity, RNF216 downregulates NF-kappa-B and IRF3 activation by promoting the degradation of TRAF3, TLR4, and TLR9. It also regulates autophagy through the ubiquitination of BECN1 and modulates synaptic strength via the degradation of ARC. Furthermore, RNF216 is essential for spermatogenesis, where it regulates meiosis by targeting PRKACB. Clinically, mutations in the RNF216 gene are well-established causes of Gordon Holmes Syndrome, a rare neurodegenerative disorder characterized by hypogonadotropic hypogonadism, ataxia, and dementia. |
检测原理