Overview
| 别名 | Aspartyl aminopeptidase |
| 基因名 | DNPEP |
| UniProt ID | Q9ULA0 |
| 反应种属 | Human,Mouse |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 5F2-M8-P5 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 53 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Aspartyl aminopeptidase (DNPEP) is a zinc-dependent metalloaminopeptidase belonging to the M18 family, characterized by its strict specificity for acidic N-terminal amino acids, specifically preferring aspartic acid over glutamic acid. The enzyme exhibits a complex dodecameric structure composed of six dimers, forming a tetrahedral arrangement with a central cavity that houses twelve active sites. Each active site contains a binuclear zinc center that coordinates substrates through a hydroxamate-like mechanism, while a restricted P1 pocket enforces the preference for acidic residues. DNPEP is primarily involved in the intracellular processing of peptides, contributing to the final stages of protein degradation and the recycling of amino acids. Its highly specialized catalytic mechanism and structural organization allow for precise control over the cleavage of peptide bonds at the N-terminus of its substrates. |
检测原理