Overview
| 别名 | LIM and calponin homology domains-containing protein 1 |
| 基因名 | LIMCH1 |
| UniProt ID | Q9UPQ0 |
| 反应种属 | Human |
| 应用 | WB,IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 1D8-Z9-W6 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 121 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:4000; IHC-1:100-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | LIM and calponin homology domains 1 (LIMCH1) is an actin stress fiber-associated protein characterized by an N-terminal calponin homology (CH) domain, a C-terminal LIM domain, and central coiled-coil regions. The CH domain facilitates actin binding, while the LIM domain mediates protein interactions. LIMCH1 regulates the activity of non-muscle myosin IIa (NM-IIA) by promoting phosphorylation of myosin regulatory light chain (MRLC), enhancing actomyosin contractility and stress fiber assembly. This function stabilizes focal adhesions and modulates cell morphology and motility. LIMCH1 depletion reduces MRLC phosphorylation, disrupts stress fibers, and decreases focal adhesion stability, leading to increased cell spreading and migration, indicating its role in restricting cell motility. In disease contexts, alterations in LIMCH1 expression may influence cancer cell migration and metastasis. |
检测原理