Overview
| 别名 | E3 ubiquitin-protein ligase TRIM35; Hemopoietic lineage switch protein 5 |
| 基因名 | TRIM35 |
| UniProt ID | Q9UPQ4 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2a |
| 克隆号 | 6P5-H5-Z8 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 56 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:500 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Tripartite motif containing 35 (TRIM35) is an E3 ubiquitin-protein ligase that plays a dual role in regulating innate immunity and cellular metabolism. In the immune system, it promotes type I interferon production by mediating the Lys-63-linked polyubiquitination of TRAF3 via RIG-I signaling, while simultaneously suppressing TLR7/9 pathways through the Lys-48-linked degradation of IRF7. It also targets viral proteins, such as influenza PB2, for degradation. Metabolically, TRIM35 reduces glucose metabolism and cell growth by inhibiting FGFR1-dependent phosphorylation of PKM. Clinically, TRIM35 functions as a tumor suppressor in several malignancies; it induces apoptosis in breast cancer by ubiquitinating PDK1 and is involved in the suppression of diffuse large B-cell lymphoma. Its diverse regulatory functions highlight its importance in maintaining cellular homeostasis and defending against viral infection and oncogenesis. |
检测原理