Overview
| 别名 | 1-phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type III PIP kinase; Serine-protein kinase PIKFYVE |
| 基因名 | PIKFYVE |
| UniProt ID | Q9Y2I7 |
| 反应种属 | Human,Mouse |
| 应用 | IHC-P,ELISA |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2b |
| 克隆号 | 3M7-U6-C7 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 237 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:200; ELISA-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | Phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE) is an enzyme encoded by the PIKFYVE gene in humans, which plays a crucial role in phosphoinositide metabolism. It primarily phosphorylates phosphatidylinositol 3-phosphate (PtdIns3P) to produce phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 5-phosphate (PtdIns5P). PIKFYVE is a large protein with multiple functional domains and is expressed in several spliced forms. Its enzymatic activity is essential for vesicular dynamics, affecting trafficking pathways through the endosomal system. Impaired PIKFYVE activity leads to lysosome enlargement and cytoplasmic vacuolation due to disrupted PtdIns(3,5)P2 synthesis and lysosome fission. Mutations in PIKFYVE are associated with Francois-Neetens corneal fleck dystrophy and have been implicated in pathogen invasion, including HIV and Salmonella replication, as well as type 2 diabetes through the inhibition of insulin-regulated glucose uptake. PIKFYVE also interacts with other proteins to form a complex that tightly controls PtdIns(3,5)P2 levels, highlighting its importance in cellular processes. |
检测原理