Overview
| 别名 | Probable E3 ubiquitin-protein ligase HECTD4; HECT domain-containing protein 4; HECT-type E3 ubiquitin transferase HECTD4 |
| 基因名 | HECTD4 |
| UniProt ID | Q9Y4D8 |
| 反应种属 | Human |
| 应用 | WB |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG1 |
| 克隆号 | 8U7-T2-D4 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 439 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | WB-1:1000 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | HECT domain E3 ubiquitin protein ligase 4 (HECTD4) is a large enzyme over 4000 amino acids long with multiple isoforms, functioning as an E3 ubiquitin-protein ligase. It facilitates the transfer of ubiquitin from an E2 enzyme to substrate proteins, a key step in ubiquitination that tags proteins for degradation or regulates their activity, thus playing important roles in protein quality control and cellular regulation. HECTD4 belongs to the HECT family characterized by a catalytic HECT domain responsible for its ubiquitin ligase activity. Functionally, it is implicated in diverse cellular processes including glucose homeostasis and protein modification within the endoplasmic reticulum. Clinically, biallelic variants in HECTD4 have been associated with neurodevelopmental disorders overlapping with Angelman syndrome. Such mutations disrupt ubiquitination pathways, leading to impaired protein homeostasis and contributing to the disease phenotype. |
检测原理