徐州威格顿生物科技有限公司
威格顿生物
Weigedun Biotechnology
BUB1 Mouse mAb
规格信息
| 规格 | 货号 |
|---|---|
| 50ul | WGD-Z-2611011 |
| 100ul | WGD-Z-2611011 |
详细参数
| 别名 | Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A |
| 基因名 | BUB1 |
| UniProt ID | O43683 |
| 反应种属 | Human |
| 应用 | IHC-P |
| 宿主 | Mouse |
| 偶联物 | Unconjugated |
| 修饰 | Unmodified |
| 亚型 | IgG2b |
| 克隆号 | 1G1-J2-U1 |
| 克隆性 | Monoclonal Antibody |
| 分子量 | Calculated MW: 122 kDa |
| 纯化方式 | Affinity Purified |
| 产品形式 | Liquid |
| 推荐稀释比 | IHC-1:200 |
| 存储缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% sodium azide |
| 保存温度 | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| 背景信息 | BUB1 mitotic checkpoint serine/threonine kinase (BUB1) is a central regulator of the spindle assembly checkpoint (SAC), essential for ensuring proper chromosome alignment and segregation during mitosis. It localizes to outer kinetochores where it recruits key checkpoint proteins, including Mad1, Mad2, and BubR1, and phosphorylates histone H2A to facilitate the recruitment of Shugoshin for centromeric cohesion protection. BUB1 also interacts with Cdc20 to regulate the timing of the metaphase-to-anaphase transition. While its catalytic activity supports SAC strength, the physical presence of the protein is critical for maintaining genomic stability. Clinically, dysregulation or mutation of BUB1 is well-linked to chromosomal instability and aneuploidy, which are hallmarks of cancer progression. Overexpression of BUB1 is frequently observed in various malignancies, including colon and lung cancers, where it serves as a driver of mitotic errors and a potential target for SAC-directed therapies. |